High Resolution Episcopic Microscopy (HREM) is a 3D destructive imaging technique comprising of 2D sections for structural analysis of samples.
Summary of the benefits of the HREM imaging technique:
-
Produce 1-6 micron complete voxel resolution over an entire volumetric dataset.
-
Image dense tissue and bone at high contrast without variation.
-
No sample clearing, sample clearing or interpolation.
-
Optical HREM systems are capable of imaging up to 60mm samples.
What is High Resolution Episcopic Microscopy (HREM)?
High-Resolution Episcopic Microscopy (HREM) is a 3D imaging technique for visualising samples ranging from 1-30mm. Resin mounted samples are stained, then sectioned repeatedly with the remaining block face imaged, creating high-resolution stacks at 1–8-micron voxel resolution.
We offer two systems for performing HREM, the Micro and Ultra. As well as other products.
Advantages of High Resolution Episcopic Microscopy (HREM)
Flexibility for a Range of Samples
High-Resolution Episcopic Microscopy (HREM) delivers exceptional clarity and resolution, even when imaging denser tissues or tissues with varying opacities.
Unlike conventional techniques, eliminate the need for tissue clearing and interpolation, making the imaging process more efficient and convenient. HREM produces high-contrast results, enhancing the visualization of anatomical structures and details.
-
Capable of visualising samples up to 30mm in one capture or greater with scanning.
-
Suitable for bone and denser tissue samples.
-
High contrast images even in higher density areas.
High Resolution for All Tissue and Sample Types
High-Resolution Episcopic Microscopy (HREM) offers a variety of resolutions with scanning stages and a choice of optics. It can capture single shots or high-resolution scans at 100x magnification with blending, no blocking.
With sections as thin as 1 µm, from our speciality section system, HREM achieves voxel resolutions of 1-8 µm³, enabling the identification of fine details like individual nerves and blood vessels, surpassing other techniques.
-
1-8 micron voxel size resolution.
-
100x magnification.
-
XY scanning stage integrated into the software available for larger samples.
Easy Data with HREM
High-Resolution Episcopic Microscopy (HREM) data is readily applicable for both 2D and 3D analysis. Simple software packages like Fiji and Dragonfly can be used to generate impressive images by inverting the LUT (Lookup Table).
HREM images can be manually or automatically segmented in the user-friendly 2-D image format, enabling precise measurements.
-
Data is stored as image files for easy manipulation (jpeg,tif and more).
-
Stored image sizes for easy workflow.
-
No custom image analysis software required.
Applications for HREM
High Resolution Episcopic Microscopy (HREM) is used in the study of:
-
Whole Mouse and Zebrafish embryos.
-
and much more.
As the field of HREM continues to expand, new research papers emerge each year, showcasing the potential in different applications.
Integrate into your research, gaining a valuable tool in your workflow. Explore the accomplishments of our satisfied HREM customers by delving into their published papers.
Optical HREM Models
We have two Optical HREM instruments designed for 3D imaging of samples with the highest quality and flexibility for users.
Optical HREM Micro
The Micro is a simple, compact Optical HREM system designed for smaller user groups and demand. Ideal for individual labs or users wanting to try HREM samples.
-
Single sample capture up to 25mm FOV.
-
20 Megapixel image output (varying on application).
-
One standard HREM channel.
-
Smaller frame, no adjustable XY (manual or automatic).
Optical HREM Ultra
Ultra systems showcase the Optical HREM systems designed for the more demanding of images, featuring varying addons to increase resolution and productivity.
-
Sample holder with up to 4 blocks at once (more for smaller blocks upon request).
-
Up to 8 light channels (multiple filters etc).
-
A scanning stage for easier mobility and scanning of samples.
-
Larger frame for all of the addons and features.
3D Volume Viewer for HREM Volumes
We offer a free 3D volume rendering software for HREM images but will of course work on any volume stack.
The viewer can view all samples from HREM such as hearts, full embryos and more. With the option to resize datasets, add contrast and more.
Features will be added but this utility will allow users to share and show datasets easily.
Contact our High-Resolution Episcopic Microscopy (HREM) experts
Want to know more about HREM, ask for a quote or get questions answered. Contact us and we can help answer all your questions.
Phone:
+44(0) 1462633500
Email:
hello@indigo-scientific.co.uk