A system that produces stacks of successive, high-quality, 2D images - perfectly aligned to create superbly detailed 3D models for morphological studies.
Available globally from Indigo Scientific
Customer HREM 3Ds
description Research papers
A listing of research papers that have utilised Optical HREM (high resolution episcopic microscopy) imaging in various fields of scientific research over the last 15 years.
HREM consumables
We have a range of HREM consumables available to buy, such as moulds, bases, sample vices and holders. Contact us to place an order, or for pricing or further information.
Get in touch3D Optical HREM User Group
Anyone with an interest in using 3D Optical HREM can join the new HREM user group on Slack.
Already a member?
There are three main steps in 3D Optical HREM imaging: embedding, sequential imaging and visualisation.
3D Optical HREM imaging is suited to a range of applications, including mouse, chicken, plant, fly and bone.
Single channel HREM
Standard HREM mode is single-channel.
Dual & multi-fluorescence HREM
HREM is also suited to dual and multiple fluorescence.
help Frequently asked questions
We've collated some more information about Optical HREM (high resolution episcopic microscopy) imaging in an FAQ.
What our customers say chat
"HREM data has allowed the DMDD team to identify nearly 400 different phenotypes in knockout mouse embryos. These range from large-scale organ malformations to the abnormal development of nerves and blood vessels."
Resolution
Constant resolution through sample depth
Sections can be cut as thin as 1 μm, which results in 3D models with a typical voxel resolutions of 1-8 μm3. This resolution enables identification of blood vessels and nerves, often not visible using lower-resolution techniques such as optical projection tomography (OPT), micro-magnetic resonance imaging (μMRI) or micro-computed tomography (μCT).
No sample distortion
Sample sizes up to 30 mm
Samples are embedded in a hard plastic resin that enables sections from 1 - 10 microns to be accurately removed.
Optical imaging
Flexible, optical-based system
A felixible, optical-based system, suitable for use over a broad range of magnifications that can be configured for multiple fluorescent channels.
Applications
Most tissue types and bone possible
Ideal for developmental biology, and used in laboratories world-wide for 3D imaging of mouse embryos, embryonic organs, different tissue types and even plants.
Alternate views
Orthogonal and oblique views
Image data produced can be used to calculate orthogonal and oblique views of the tissue sample, with little resolution lost. These views can provide important additional information about sample morphology.
people Our customers
Our 3D Optical HREM imaging systems are used by customers around the world, including universities, institutes and biotech companies.