Who Manufactures High Resolution Episcopic Microscopy (HREM)

Optical HREM is designed and manufactured by Indigo Scientific (i-scientifc.com)

Can High Resolution Episcopic Microscopy (HREM)be adapted to new techniques,

High Resolution Episcopic Microscopy (HREM) is flexible and we can help adapt the system to user requirements, get in contact with Indigo Scientific to see what we can do.

What is the difference between HREM and OHREM

HREM and OHREM are interchangeable however at Indigo we refer to OHREM as the system and HREM as the Technique. OHREM stands for Optical High Resolution Episcopic Microscopy.

Do you ship HREM globally?

Yes we ship and service High Resolution Episcopic Microscopy (HREM) globally, service contracts are provided to help maintain your m

How does High Resolution Episcopic Microscopy (HREM) work.

OHREM works by using a custom made microtone made by Indigo Scientific to section a sample. The sample is imaged during sectioning giving an image series often stained specifically to look at anatomy however, not limited to.

What samples can be run on High Resolution Episcopic Microscopy (HREM)?

theory it is down to the staining but already there has been great success in a variety of different specimens, we can offer interested parties imaging of there samples after following the protocol found in the members area. Get in touch to find out more.

Maximum Size Sample for High Resolution Episcopic Microscopy (HREM)

A single shot optic (no scanning) is limited to the optical limit of 30mm for the standard system. However, with changes to the configuration the OHREM can achieve success of samples of 60mm wide. Get in touch to find out more.

How many High Resolution Episcopic Microscopy (HREM) samples can be imaged at once

Revision 1 OHREM systems can only image 1 block per section. OHREM 2 and an adapted OHREM 1 is capable of imaging 2 20 mm blocks or 4 10 mm blocks with the facility to expand this if required. It is worth noting a block can contain more than one sample, for example one block can contain 3 embryonic mouse hearts.

High Resolution Episcopic Microscopy(HREM) instrument with Heart background

High Resolution Episcopic Microscopy (HREM) System

High Resolution Episcopic Microscopy (HREM) is a 3D destructive imaging technique comprising of 2D sections for structural analysis of samples.

Summary of the benefits of the HREM imaging technique:

Produce 1-6 micron complete voxel resolution over an entire volumetric dataset.
Image dense tissue and bone at high contrast without variation.
No sample clearing, sample clearing or interpolation.
Optical HREM systems are capable of imaging up to 60mm samples.

High Resolution Episcopic Microscopy(HREM) Cross Sectional Image for 3D data

What is High Resolution Episcopic Microscopy (HREM)?

High Resolution Episcopic Microscopy (HREM) is a 3D imaging technique for visualising samples ranging from 1-30mm. Resin mounted samples are stained, then sectioned repeatedly with the remaining block face imaged, creating high-resolution stacks at 1–8-micron voxel resolution.

Advantages of High Resolution Episcopic Microscopy (HREM)

High Resolution Episcopic Microscopy (HREM) Mouse Head Image

Flexibility

Image a wide range of samples and density’s with Optical HREM instruments. Image varying density’s and tissue types, including plant matter, with surface based imaging.

Resolution

Output of 1-8 micron voxel resolution gives the flexibility to visualise detail unseen in other techniques and systems.

Easy Data

Optical HREM provides straight forward images as an output with no processing. Captured in 2D stacks easily converted into 3D volume renderings.

Adaptable

High-resolution episcopic microscopy (HREM) as a technique requires no sample clearing or interpolation. This opens up the floor to 3D imaging samples in detail that previously could not be imaged in such detail.

Flexibility for a Range of Samples

High-Resolution Episcopic Microscopy (HREM) delivers exceptional clarity and resolution, even when imaging denser tissues or tissues with varying opacities.

Unlike conventional techniques, eliminate the need for tissue clearing and interpolation, making the imaging process more efficient and convenient. HREM produces high-contrast results, enhancing the visualization of anatomical structures and details.

Capable of visualising samples up to 30mm in one capture or greater with scanning.
Suitable for bone and denser tissue samples.
High contrast images even in higher density areas.

High Resolution Episcopic Microscopy (HREM)

High-Resolution Episcopic Microscopy Cross Section

High Resolution for All Tissue and Sample Types

High-Resolution Episcopic Microscopy (HREM) offers a variety of resolutions with scanning stages and a choice of optics. It can capture single shots or high-resolution scans at 100x magnification with blending, no blocking.

With sections as thin as 1 µm, from our speciality section system, HREM achieves voxel resolutions of 1-8 µm³, enabling the identification of fine details like individual nerves and blood vessels, surpassing other techniques.

1-8 micron voxel size resolution.
100x magnification.
XY scanning stage integrated into the software available for larger samples.

High-Resolution Episcopic Microscopy Mouse Embryo

Easy Data with HREM

High-Resolution Episcopic Microscopy (HREM) data is readily applicable for both 2D and 3D analysis. Simple software packages like Fiji and Dragonfly can be used to generate impressive images by inverting the LUT (Lookup Table).

HREM images can be manually or automatically segmented in the user-friendly 2-D image format, enabling precise measurements.

Data is stored as image files for easy manipulation (jpeg,tif and more).
Stored image sizes for easy workflow.
No custom image analysis software required.

Applications for HREM

High Resolution Episcopic Microscopy (HREM) is used in the study of:

Mouse organs (such as brains and hearts).
Whole Mouse and Zebrafish embryos.
Plant matter.
Drosophila.
and much more.

As the field of HREM continues to expand, new research papers emerge each year, showcasing the potential in different applications.

Integrate into your research, gaining a valuable tool in your workflow. Explore the accomplishments of our satisfied HREM customers by delving into their published papers.

View Examples

View Applications

How does the Optical HREM system work?

High Resolution Episcopic Microscopy (HREM) is a unique imaging technique that enables three-dimensional visualization of various samples in 2D image sections.

Optical HREM systems consists of a precise, custom-made microtome capable of consistently sectioning samples, embedded in resin, in sections ranging from 1 to 10 microns. At each pass, the block-face is imaged, and by combining these two-dimensional images, volumetric data is generated to construct a comprehensive three-dimensional stack.

The instruments accommodate samples up 30mm along the Z-axis and even larger dimensions along the X and Y axes (upon request). The samples can range from millimetres to the full extent of 30mm. OHREM can capture high-resolution, clear images regardless of the tissue type or the developmental stage, even when dealing with opaque or translucent tissues.

Optical High Resolution Episcopic Microscopy Models

High Resolution Episcopic Microscopy (HREM) Micro

HREM Ultra

Optical HREM ultra for large scale imaging with higher resolution up to 30mm samples.

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HREM Micro

OHREM micro for imaging samples up to 25mm simply. The smaller system to the ultra for individual users.

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HREM Consumables and Accessories

High Resolution Episcopic Microscopy (HREM) Ultra

Split Mould for Embedded Samples

Heat resistant moulds designed to withstand high thermal reactions for HREM samples. Splits in half allowing for taller blocks to be made.

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Resin Chuck for Mounted Samples

HREM Chucks for resin samples up to 25mm. Contact for more information or a quote.

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HREM Imaging Systems produce simple datasets that can be used in almost any 3D packages such as Dragonfly or even ImageJ/Fiji. Here at Indigo we make some packages to help use the machine as well as manage some datasets specially designed for block face imaging/HREM.

OHREM Acquire

OHREM Acquire is the custom software specially designed for HREM imaging, this software has been streamlined to help users achieve all levels of features without complexity. Acquire was designed in house by our software engineers to meet the needs of the users.

Acquire reduces excessive time delays by optimizing transitions, for example combining filter movement operations and XY movement operations to reduce experiment times.

Manual control of the Indigo Scientific instruments can be achieved with acquire, allowing you to complete standard operations without extra equipment.

Acquire features a bespoke stitching algorithm for HREM images allowing perfectly Z aligned images, with blending. This is done without blocking and long and repetitive calculations. The output are amazing 2D images ready for use in any existing 3D software package such as Dragonfly or Fiji.

At Indigo Scientific we believe in giving software for the length of the time of the machine. With no rolling licenses but optional upgrades and updates included in service contract. Along with this we will listen and put in custom features from all user requests.

OHREM Utilities

OHREM Utilities is a free windows application available to all for simple processing and visualisation of HREM datasets. This application allows users to batch process datasets easily.

In built stitching, with an auto stitcher for smaller mosaics and a custom stitcher for larger datasets with unique blending and alignment for block face imaging. With tools for stacks such as alignment and merging of separate stacks.

Simple image pipeline editor for stacks with CLAHE, LUT inversion and basic thresholding for segmentation.

Testimonials

"HREM data has allowed the DMDD team to identify nearly 400 different phenotypes in knockout mouse embryos. These range from large-scale organ malformations to the abnormal development of nerves and blood vessels."

Tim Mohan
(DMDD)

Want to know more?

Brochure

Contact our High-Resolution Episcopic Microscopy (HREM) experts

Want to know more about HREM, ask for a quote or get questions answered. Contact us and we can help answer all your questions.

Phone:
+44(0) 1462633500

Email:
hello@indigo-scientific.co.uk

Phone: +44(0) 1462633500

Email: hello@indigo-scientific.co.uk

High Resolution Episcopic Microscopy (HREM) System

What is High Resolution Episcopic Microscopy (HREM)?