We've collated some information, that may help answer some of your initial questions. Please get in touch if you'd like any further information.
Frequently asked questions
- 3D Optical HREM imaging provides a way to view structures and abnormalities that may not be possible using different methods.
- No distortion - embedding samples in a hard resin enables the block face to be imaged.
- Superb detail - sections can be cut as thin as 1 μm, enabling individual blood vessels and nerves to be identified.
- Speed - clearing is not needed, neither do samples need to be collected, or registered, or stained. This enables, for example, an E14.5 mouse embryo to be be sectioned in approximatley 8 hours, producing around 3,000 images.
- Perfectly aligned images - because the images are cut from the block face, they are accurately aligned, ready to build detailed 3D models, which can also provide orthogonal and oblique views.
3D Optical HREM Imaging is suited to the examination of a huge range of embryos, organs and tissues. There are two main considerations - sample size and sample hardness:
- It's generally suited to samples up to 2cm.
- For very hard samples like bones, teeth etc, penetration of the JB4 may require a longer infiltration time. Also, samples can not be harder than fully backed JB4. Once these two requirements are met, cutting should be possible.
The only other considerations are the camera and optics being used, and this is where the expertise from Indigo Scientific will support you.