High Resolution Episcopic Microscopy (HREM) Applications

Image a Variety of Samples with Optical HREM Instruments

Overview of HREM Applications

HREM technique was originally derived for the visualisation of neurological, cardiovascular, skeletal, and abnormalities across diverse mouse models. Beyond this HREM has grown to find further applications due to the benefits it provides over other techniques and technologies.

Cross section 2D images of a mouse sample imaged with optical HREM block face imaging systems combined together for a high-resolution 3D volume of anatomy.

Benefits of HREM for 3D Imaging

HREM is a powerful 3D imaging technique that combines fluorescence illumination with block face imaging for high detail, histological resolution 2D images that combine for full 3D images.

Versatile Image Sizes

HREM offers a versatile imaging platform with resolutions down to sub-micron in the X and Y and up to 1 micron in the Z. While also allowing images of larger structures up to 30mm.

Compatible with a Wide Range of Samples

Suitable for embryos, organs, plant structures and more.

Consistent Imaging Quality

Provides reliable and reproducible 3D datasets that can be used for high throughput applications such as phenotyping and comparative studies.

Consistent Imaging Quality

There is no requirement for clearing of samples which avoids tissue shrinkage.

HREM System Applications

Applications commonly, but not limited to, for HREM imaging. Perform 3D imaging of mouse hearts, barley, whole embryos and more with this cost effective 3D imaging technique. Click here to view HREM examples such as 3D or 2D images.

Agricultural Samples (Plant & Crop Science)

High-resolution episcopic microscopy (HREM) image of a plant sample (barley), revealing intricate internal structures and cellular organization in 3D. The grayscale rendering highlights detailed tissue morphology

HREM has been used to study a variety of samples within the agricultural sector these include barley, citrus fruit, root structure analysis and more.

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Heart and Cardiovascular Research

Mouse embryo heart captured in high resolution using HREM imaging system for 3D data for CHD research.

Capture the intricate details of heart chamber morphology and valve structures with HREM. Used for detecting subtle developmental abnormalities in embryos and adult mice.

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Whole Embryo Imaging & Developmental Biology

HREM Mouse Embryo Sample captured in 3D with Optical HREM system for mass phenotyping.

Capture entire embryo in 3D with sub-micron resolution, ideal for morphological studies and birth defect research. Enables large scale phenotyping of genetic mutants.

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Whole Imaging of Drosophila & Invertebrates

Drosophila Sample Imaged with Serial Block Face Imaging showcasing HREMs use in insect 3D imaging at high resolution.

Visualise fly’s or other common organisms often used for genetic studies in 3D.

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Serial Block-Face Imaging

An upright reflected light microscope imaging in florescence on an optical hrem ultra system.

Use Optical HREM instruments to perform block face imaging to create high detail 3D data with either resin or other mediums. For performing EFIC or other techniques as well as HREM for contrast with modular and swappable instruments.

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3D Imaging for Various Applications

Optical HREM micro showcasing imaging of a mouse embryo with block face imaging in reflected fluorescence. For high resolution with low cost.

Optical HREM Instruments can be used beyond this application to observe a variety of samples including samples containing bone in 3D. Why not contact us for more information?

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Contact our High-Resolution Episcopic Microscopy (HREM) Experts

Want to know more about HREM or if its compatible with your application, ask for a quote or get questions answered. Contact us and we can help answer all your questions.

Phone:
+44(0) 1462633500

Email:
hello@indigo-scientific.co.uk