HREM Ultra

Flagship Optical HREM system for performing sectioning and imaging of larger, denser biological samples. Visualise small detail in larger samples with the Optical HREM Ultra.

Learn more about High Resolution Episcopic Microscopy here, if you would like to know more about the Ultra imaging system contact us.

Overview

Ultra allows users to image a range of samples and experiment with different types of imaging. This serial section imaging system provides the perfect tool for a whole range of ideas and opportunities from fluorescent dyes to sample size and quantity.

Features

• Image Uncleared, Dense, Samples – One of the biggest benefits of HREM and therefore the ultra is the ability to image whole dense structures.

• Mass Histology Imaging – With the option to combine samples into one holder, users can image up to 4 samples in one run. Not only does this allow for time-saving but allows for easy comparison of samples.

• High-Resolution – Single shot can capture 20 megapixels but can be scanned to achieve large resolution images of samples using the custom built XY stage with Z resolution down to 1 micron. Giving perfectly aligned 3D stacks of high quality captures.

Applications

The ultra can be used for a whole variety of applications for volumetric reconstruction but can especially be used for:

• Cardiovascular Research – Ultra systems can be used to image the complex cardiovascular system of mice and other species with great detail and resolution. The ultra provides greater XY resolution in the individual image stack over revision 1 systems and the micro, with the use of scanning.

• Developmental Biology – Capture a wide range of samples in the field, from plant matter, mouse embryos and pups, zebrafish and more. With the larger platform capture more developed samples at greater densities and size.

• Plant Sciences – Image whole plant structures in 3D including barley and citrus fruit.

• Many more – Our serial sectioning imaging system could be used to image much more, let us know your application.

Specification

The standard Ultra configuration consists of the following:

• Optics - Optical platform with a maximum field of view of up to 25 x 25mm, with a variable zoom optic. An upright microscope can be added to the setup instead of a variable zoom microscope.

• XY Optic Control – An automated custom made XY stage is fitted as standard to the ultra for scanning, multi sample capture and XY positioning. With this all being included in the software for the lifetime of the instrument. The XY has a position limit of 100mm X and Y*

• Electronic Focus - Electronic focus for controlling focus via software (Zoom microscopes only).

• Channels - 1 Morphology channel illumination as standard for imaging traditional HREM but can be adapted to suit.

• Software - Included OHREM Acquire capture software, no license and free support. With many layers of automation and features to streamline and improve workflow. As well as a whole array of free tools for stitching images, aligning stacks and viewing stacks.

• Sample holders - Comes standard with 1 position sample holder with grips for extra sample stability.

• Blades - Compatible with both 60, 80mm and 160mm resin blades for a variety of sectioning needs.

• Imaging - 20 Megapixel monochrome image output for high resolution analysis. This can be adapted upon request.

• Sample Extraction - Extraction unit for sample removal, this avoids debris remaining fixed to the sample.

• Encoder - An encoder for exact section thickness information for reconstruction.

• Warranty - 1 year warranty with access to parts and our team.

• Training - On site training for one or two full days, along with a year ‘getting it up and running’ help as you start to produce wonderful 3D datasets.

• Consumables and Preparation - 1 Split mould for resin HREM samples custom made along with 20 chucks for HREM samples.

• PC and Setup - PC setup with all software and drivers prior to install so you are ready to go.

*May be limited by hardware.

Add-ons and extras

These add-ons and extras can be fitted after the machine is installed or purchased with the unit.

• Multiple Illumination Channels - More than 1 illumination channel and filter set, this can include filter wheels/sliders. For imaging different fluorescence channels. Up to 8 controllable channels and intensity’s, adaptable in the acquire software. This setup also allows for filter sliders/wheels for a combination of filters.

• Imaging - Ultra can be fitted with different imaging setups, upon request. Higher resolutions, more sensitivity, colour or monochrome. As well as adaptions to the the optical setup to allow for compound microscopes instead of zoom optics.

• Multi Sample Holder – A multi well sample holder for holding 2,4 or a custom sample/s, this helps increase throughput of the serial imaging. Available for individual sale or as a kit.

• Z Height Increase – With modification, the unit can achieve 50mm in Z height for larger samples. This cannot be added onto existing units.

• Have an idea? – We do offer the ability to adapt each unit to specific needs. Such as if you wanted to use some extra piece of technology we can help. Adding features to software or hardware to suit your imaging needs.

• Consumables – We offer quality split moulds custom made to sizes you require as well as chucks to fit them.

• Service Contract and Warranty’s – We offer an all or nothing service contract and warranty. Meaning you are covered for all issues on the unit, and we will be here to help you get up and running as soon as we can. Conditions do apply.