A system that produces stacks of successive, high-quality, 2D images - perfectly aligned to create superbly detailed 3D models for morphological studies.

Available globally from Indigo Scientific

Customer HREM 3Ds

description Research papers

A listing of research papers that have utilised Optical HREM (high resolution episcopic microscopy) imaging in various fields of scientific research over the last 15 years.

HREM consumables

We have a range of HREM consumables available to buy, such as moulds, bases, sample vices and holders. Contact us to place an order, or for pricing or further information.

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3D Optical HREM User Group

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Anyone with an interest in using 3D Optical HREM can join the new HREM user group on Slack.

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3D Optical HREM imaging has been widely used to identify phenotypes in embryo heart development.

A range of applications and models, including embryo heart development, craniofacial development, bone tissue, mouse, chicken, plant, fly, and more, can be imaged and visualised using 3D Optical HREM imaging.

There are three main steps in 3D Optical HREM imaging: embedding, sequential imaging and visualisation.

Single channel HREM

Standard HREM mode is single-channel.

Dual & multi-fluorescence HREM

HREM is also suited to dual and multiple fluorescence.

help Frequently asked questions

We've collated some more information about Optical HREM (high resolution episcopic microscopy) imaging in an FAQ.

What our customers say chat

"HREM data has allowed the DMDD team to identify nearly 400 different phenotypes in knockout mouse embryos. These range from large-scale organ malformations to the abnormal development of nerves and blood vessels."


Constant resolution through sample depth

Sections can be cut as thin as 1 μm, which results in 3D models with a typical voxel resolutions of 1-8 μm3. This resolution enables identification of blood vessels and nerves, often not visible using lower-resolution techniques such as optical projection tomography (OPT), micro-magnetic resonance imaging (μMRI) or micro-computed tomography (μCT).


No sample distortion

Sample sizes up to 30 mm

Samples are embedded in a hard plastic resin that enables sections from 1 - 10 microns to be accurately removed.


Optical imaging

Flexible, optical-based system

A felixible, optical-based system, suitable for use over a broad range of magnifications that can be configured for multiple fluorescent channels.



Most tissue types and bone possible

Ideal for developmental biology, and used in laboratories world-wide for 3D imaging of mouse embryos, embryonic organs, different tissue types and even plants.


Alternate views

Orthogonal and oblique views

Image data produced can be used to calculate orthogonal and oblique views of the tissue sample, with little resolution lost. These views can provide important additional information about sample morphology.

people Our customers

Our 3D Optical HREM imaging systems are used by customers around the world, including universities, institutes and biotech companies.

Find out more about 3D Optical HREM

We’d be delighted to give you further information and find out if it’s the right solution for you.

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